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Literature summary extracted from

  • Horn, C.; Sohn-Boesser, L.; Breed, J.; Welte, W.; Schmitt, L.; Bremer, E.
    Molecular determinants for substrate specificity of the ligand-binding protein OpuAC from Bacillus subtilis for the compatible solutes glycine betaine and proline betaine (2006), J. Mol. Biol., 357, 592-606.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.6.2.9 substrate-binding domain into vector pBKB76, expression in Escherichia coli BL21 (DE3) Bacillus subtilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
7.6.2.9 by hanging-drop method, structures of substrate-binding domain in complex with glycine betaine solved at 2.0 A resolution and in complex with glycine proline at 2.8 A resolution, structures show a substrate-binding protein-dependent-typical class II fold, structural differences of complexes occur within the ligand-binding pocket as well as across the domain-domain interface, explaining the differences in affinity of the substrate-binding domain-glycine betaine complex with KD = 0.017 mM, and substrate-binding domain-proline betaine complex with KD = 0.295 mM Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.6.2.9 membrane
-
 Bacillus subtilis 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.6.2.9 28000
-
 substrate-binding domain, 1 * 28000, SDS-PAGE Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
7.6.2.9 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.6.2.9 substrate-binding domain, to apparent homogeneity Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.6.2.9 ATP + H2O + glycine betaine/out
-
 Bacillus subtilis ADP + phosphate + glycine betaine/in
-
 ?
7.6.2.9 ATP + H2O + proline betaine/out
-
 Bacillus subtilis ADP + phosphate + proline betaine/in
-
 ?

Subunits

EC Number Subunits Comment Organism
7.6.2.9 monomer substrate-binding domain, 1 * 28000, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
7.6.2.9 OpuA
-
 Bacillus subtilis